rMutarotase 

rMutarotase 
Mutarotase (Recombinant from Pig kidney)
組換えムタロターゼ (ブタ腎臓由来)

反応

α-D-Glucose ⇄ β-D-Glucose

規格

Specific activity
IU/mg protein > 170 units
Contaminants
Lactate dehydrogenase < 0.1%
NADH oxidase < 0.01%
Glucose oxidase < 0.01%
Catalase < 1.0%

分析方法

I. Spectrophotometric Method
Wavelength: 340 nm, Light path length: 1 cm
Final volume: 3.03 mL, Temperature: 25゚C

Pipette the following reagents into a cuvette

1.50 mL
Tris-HCl buffer (0.1 mol/L, pH 7.2)
0.60 mL
NAD-Na (10 mmol/L)
0.06 mL
ATP (10 Glucose dehydrogenase (1,000 IU/mL)mmol/L)
0.80 mL
Distilled water
0.05 mL
α-D-Glucose
0.02 mL
rMutarotase (approx. 3 IU/mL)
 

II. Calculation

IU/mL =
ΔA/min・V・D

6.3・d・v

 

ΔA/min :
The change in absorbance at 340 nm/minute(reverse the blank activation of rMutarotase(-))
V :
Total volume of reaction mixture (3.03 mL)
D :
Enzyme dilution factor
6.3 :
mmol/L extinction coefficient of NADH (L・mmol-1・cm-1)
d :
Light path length (1 cm)
v :
Volume of enzyme sample (0.02 mL)
基礎データ

形態/保存

Product Code:rMutarotase-02
Ammonium sulfate suspension
Store at 1 - 10゚C

関連キーワード

体外診断薬、生化学、酵素、Glucose、グルコース、Glu

お問い合わせ

rMutarotase

包装 製品番号
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製品に関するお問い合わせ診断薬試薬/研究試薬

本品は研究用試薬であり、医薬品ではありません。