rUricase (Yeast)

rUricase (Yeast)
Uricase (Recombinant from Yeast)
組換えウリカーゼ (酵母由来)

反応

Urate + O2 + H2O ⇄ 5-Hydroxyisourate + H2O2

規格

Specific activity
IU/mg protein > 6 units

分析方法

I. Spectrophotometric Method
Wavelength: 293 nm, Light path length: 1 cm
Final volume: 3.02 mL, Temperature: 25゚C

Pipette the following reagents into a cuvette

3.00 mL
Borate buffer (50 mmol/L, pH 8.5) containing Urate (0.125 mmol/L)

 

0.02 mL
rUricase (approx. 1 IU/mL)
 

II. Calculation

IU/mL =
ΔA/min・V・D

12.6・d・v

 

ΔA/min :
The change in absorbance at 293 nm/minute (reverse the blank activation of rUricase(-))
V :
Total volume of reaction mixture (3.02 mL)
D :
Enzyme dilution factor
12.6 :
mmol/L extinction coefficient of Urate (L・mmol-1・cm-1)
d :
Light path length (1 cm)
v :
Volume of enzyme sample (0.02 mL)

 

基礎データ

形態/保存

Product Code:rUricase-03
Lyophilized powder
Store below -20゚C

関連キーワード

体外診断薬、生化学、酵素、UA、尿酸

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rUricase

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