rD-LDH (L)

rD-LDH (L)
D-Lactate Dehydrogenase (Recombinant from Leuconostoc sp.)
組換えD-乳酸脱水素酵素(Leuconostoc sp由来)

反応

D-Lactate + NAD+ ⇄ Pyruvate + NADH + H+

規格

Specific activity
IU/mg protein > 1000 units
Contaminants
Malate dehydrogenase < 0.01%
Myokinase < 0.02%
Pyruvate kinase < 0.003%
Glutamic-pyruvic transaminase* < 0.001%
Glutamic-oxaloacetic transaminase* < 0.001%

*α-Hydroxyglutarate dehydrogenase activation included.

分析方法

I. Spectrophotometric Method
Wavelength: 340 nm, Light path length: 1 cm
Final volume: 3.17 mL, Temperature: 25゚C

Pipette the following reagents into a cuvette

3.00 mL
K-phosphate buffer (0.1 mol/L, pH 7.0)
0.10 mL
Na-pyruvate (25.4 mmol/L)
0.05 mL
NADH (10 mg/mL) dissoluted in Tris (10 mmol/L)
0.02 mL
rD-LDH(L) (approx. 3 IU/mL)
 

II. Calculation

IU/mL =
ΔA/min・V・D

6.3・d・v

 

ΔA/min :
The change in absorbance at 340 nm/minute(reverse the blank activation of rLDH(PH)(-))
V :
Total volume of reaction mixture (3.17 mL)
D :
Enzyme dilution factor
6.3 :
mmol/L extinction coefficient of NADH (L・mmol-1・cm-1)
d :
Light path length (1 cm)
v :
Volume of enzyme sample (0.02 mL)
基礎データ

形態/保存

Product Code:rD-LDH(L)-83
Lyophilized powder
Store below -20゚C

関連キーワード

体外診断薬、生化学、酵素、ALT、AST、GOT、GPT

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rD-LDH(L)

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